Gene expression profile of different cell types in the tumor microenvironment of MOC1 tumors treated with Smyd3 anti-sense oligonucleotides (ASOs).. Gene expression profile of different cell types in the tumor microenvironment of MOC1 tumors treated with Smyd3 anti-sense oligonucleotides (ASOs).

We used scRNA-seq to assess the differential effect of Smyd3 ASOs on the transcriptomic profile of different cell types in the TME of MOC1 tumors. Overall design: MOC1 tumors were implanted in the flank of C57BL/6 mice were treated with control (n=3) or Smyd3 ASOs (n=3) for 18 days. Tumors were surgically resected, digested into single-cell suspensions and scRNA-seq was conducted.

本研究采用单细胞RNA测序（single-cell RNA sequencing, scRNA-seq）技术，评估Smyd3反义寡核苷酸（antisense oligonucleotides, ASOs）对MOC1肿瘤肿瘤微环境（tumor microenvironment, TME）内不同细胞类型转录组谱的差异影响。
整体实验设计如下：将MOC1肿瘤接种于C57BL/6小鼠侧腹皮下，分别以对照组（n=3）与Smyd3 ASOs处理组（n=3）干预18天。待干预结束后，手术切除肿瘤组织，将其消化制备为单细胞悬液，随后进行单细胞RNA测序（scRNA-seq）检测。