Genotype data of seedlings from pine stands renewed by direct mechanized seeding using seeds from the Salaca pine seed plantation

This is a data set of genotypes of seedlings from pine stands established by direct mechnised seeding. Seeds were obtained from the Salaca Scots pine seed plantation. DNA was extracted from needles using a modified CTAB method (Porebski et al. 1997). Genotyping was done with 16 microsatellite markers - psyl2, psyl16, psyl18, psyl25, psyl42, psyl44, psyl57, psyl17, psyl19, psyl36 (Sebastiani et al. 2012), SPAC12.5, SPAC 11.6 (Soranzo et al. 1998), PtTX2146, PtTX4011, PtTX4001, PtTX3107 (Aukland et al. 2002). PCR reaction were done in four multiplex bins – Bin1 (PtTX4011, PtTX4001, PtTX3107, psyl17, psyl19, psyl36), Bin2 (PtTX2146, SPAC12.5, SPAC 11.6), Bin3 (psyl16, psyl25, psyl44), Bin4 (psyl18, psyl2, psyl57, psyl42). The genotype data file also includes maternal assignments determined using the Cervus software (Kalinowski et al. 2007) for all stands analyzed together, as well as for each stand analyzed separately. This research was performed within the National Research Programme project No. VPP-ZM-VRIIILA-2024/2-0002 "Innovation in Forest Management and Value Chain for Latvia's Growth: New Forest Services, Products and Technologies (Forest4LV)"本数据集涵盖通过机械化直接播种营建的欧洲赤松林分幼苗的基因型数据。实验所用种子采自萨拉察（Salaca）欧洲赤松种子园。 采用改良CTAB法（CTAB method，Porebski等，1997）从针叶组织中提取基因组DNA。通过16对微卫星标记（microsatellite markers）完成基因分型，具体标记如下：psyl2、psyl16、psyl18、psyl25、psyl42、psyl44、psyl57、psyl17、psyl19、psyl36（Sebastiani等，2012）；SPAC12.5、SPAC11.6（Soranzo等，1998）；PtTX2146、PtTX4011、PtTX4001、PtTX3107（Aukland等，2002）。 聚合酶链式反应（PCR）分4组多重扩增池进行：池1（Bin1）包含PtTX4011、PtTX4001、PtTX3107、psyl17、psyl19、psyl36；池2（Bin2）包含PtTX2146、SPAC12.5、SPAC11.6；池3（Bin3）包含psyl16、psyl25、psyl44；池4（Bin4）包含psyl18、psyl2、psyl57、psyl42。 该基因型数据文件还包含基于Cervus软件（Cervus software，Kalinowski等，2007）推导得到的母本归属信息，既涵盖所有林分的联合分析结果，也包含各林分单独分析的结果。 本研究依托拉脱维亚国家研究计划项目编号VPP-ZM-VRIIILA-2024/2-0002 "拉脱维亚林业增长的森林管理与价值链创新：新型森林服务、产品与技术（Forest4LV）"开展。