RNA-seq of ex vivo differentiated osteoclasts of FLT3 ITD Ptprc KO mice and controls

The receptor tyrosine kinase FLT3 is expressed in myeloid and lymphoid progenitor cells. Here, the influence of the FLT3 ITD mutation and PTPRC inactivation on the bone remodeling was studied in mice. Gene expression analysis of differentiated osteoclasts showed dysregulated signaling pathways influencing their differentiation as well as the coupling of bone resorption and formation. Overall design: RNA-seq data comprises four osteoclast cell cultures seeded from CD11b-positve bone marrow cells of different C57BL/6J mice lines (wild type, Ptprc KO, Flt3 ITD KI, Flt3 KI Ptprc KO). 24 samples in 4 groups: wild type (4 samples), Ptprc KO (4 samples), Flt3 ITD KI (4 samples), Flt3ITD KI Ptprc KO (4 samples).

受体酪氨酸激酶FLT3（receptor tyrosine kinase FLT3）表达于髓系与淋巴系祖细胞中。本研究以小鼠为模型，探究了FLT3 ITD突变与PTPRC失活对骨重塑的影响。对分化成熟破骨细胞的基因表达分析显示，多条调控其分化以及骨吸收与骨形成耦联的信号通路存在表达失调现象。整体实验设计：RNA测序（RNA-seq）数据集包含4组破骨细胞培养物，均由不同C57BL/6J小鼠品系的CD11b阳性骨髓细胞接种制备。该数据集共设4组、24个样本：野生型组（4个样本）、Ptprc敲除（Ptprc KO）组（4个样本）、Flt3 ITD敲入（Flt3 ITD KI）组（4个样本）以及Flt3 ITD KI Ptprc KO组（4个样本）。