Nucl.eye.D

Here you can find all datasets, scripts and trained models related to the Nucl.eye.D workflow presented in the publication - "Advanced image analysis methods for automated segmentation of subnuclear chromatin domains " ! The folder nucleyed.zip contains: - trained models (>Output>models) - training data-set (>Input) - all images (>Input) The folder SupplementalMacros.zip contains all supplemental macro used for the analysis. The file : The_Nucl_Eye_D.ipynb can be directly used following the noted instructions : - Download The_Nucl_Eye_D.ipynb - upload it to your Google Drive - Open it with Google Colaboratory (Or if not already installed : Open with -> More applications -> Google Colaboratory -> Install) (We recommend using Chrome browser) With the updated from 08.04.2024 the folder "H3K9me2 and H3K27me1 segmentation models.zip" was added - containing models for segmentation of H3K9me2 or H3K27me1 immunostained nuclei. Training sets are also contained. For question about the script contact : [e-mail] Enjoy using Nucl.eye.D !本资源包含与发表于论文《用于亚核染色质结构域自动化分割的先进图像分析方法》中的Nucl.eye.D工作流相关的全部数据集、脚本与训练模型。 nucleyed.zip 压缩包内含：训练模型（存储路径：>Output>models）、训练数据集（存储路径：>Input）以及全部原始图像（存储路径：>Input）。 SupplementalMacros.zip 压缩包包含本次分析所用的全部补充宏脚本。 文件 The_Nucl_Eye_D.ipynb 可按照下述说明直接使用： - 下载 The_Nucl_Eye_D.ipynb 文件 - 将其上传至您的谷歌云盘（Google Drive） - 使用谷歌协作平台（Google Colaboratory）打开该文件；若未安装该应用，则依次选择「打开方式→更多应用→Google Colaboratory→安装」 我们推荐使用Chrome浏览器进行操作。 2024年4月8日的更新版本中新增了「H3K9me2与H3K27me1分割模型.zip」压缩包，内含用于对经H3K9me2或H3K27me1免疫染色的细胞核进行分割的模型，同时附带对应的训练数据集。 若您对脚本有任何疑问，请联系邮箱：[e-mail] 祝您使用Nucl.eye.D愉快！