Re-aligned data from UCSC 100-way vertebrate and 124-way insect exome alignments.
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This data was used to infer compensatory frameshifting mutations for "Pairs of compensatory frameshifting mutations contribute to evolution of protein-coding sequences in vertebrates and insects" article. The details on how it was obtained can be found there. In short, the exonic regions were extracted from the genomes of species from the MULTIZ alignment according to MULTIZ annotation. These exons were concatenated and aligned using MAFFT. Resulting alignment were clipped from the ends and long regions from the middle looking like random intronic misannotations in one of the species were excluded.
本数据集用于推断补偿性移码突变(compensatory frameshifting mutations),相关研究论文为《成对补偿性移码突变推动脊椎动物与昆虫蛋白质编码序列的演化》。该数据集的获取细节可参阅该论文。简言之,研究人员依据MULTIZ(MULTIZ)注释信息,从MULTIZ序列比对结果中提取各物种基因组的外显子区域;将提取得到的外显子进行拼接,并通过MAFFT(MAFFT)完成多序列比对;最终对生成的比对序列进行末端修剪,并剔除序列中间疑似某物种随机内含子注释错误的长片段区域。




