Evaluation of Host Protein Biomarkers by ELISA from Whole Lysed Peripheral Blood for Development of Diagnostic Tests for Active Tuberculosis

This dataset is used in the manuscript: <i>Evaluation of Host Protein Biomarkers by ELISA from Whole Lysed Peripheral Blood for Development of Diagnostic Tests for Active Tuberculosis.</i><br>In this study, we evaluate the diagnostic performance of nine biomarkers from our previously published biomarker qPCR validation; CALCOCO2, CD274, CD52, GBP1, IFIT3, IFITM3, SAMD9L, SNX10 and TMEM49, as protein moieties assayed by ELISA. This preliminary, pilot evaluation study was conducted to quantify the level of biomarker protein expression across latent, extra-pulmonary or pulmonary TB groups and negative controls, collected across the UK and India, in whole lysed blood samples (WLB). <br><br>

本数据集用于以下学术手稿：《通过ELISA检测全裂解外周血宿主蛋白生物标志物以开发活动性结核病诊断检测方法》。本研究针对我们此前经定量聚合酶链反应（qPCR）验证的生物标志物中的9种蛋白展开评估，即CALCOCO2、CD274、CD52、GBP1、IFIT3、IFITM3、SAMD9L、SNX10及TMEM49，以ELISA法检测其蛋白形式的表达水平。本次初步预实验研究旨在定量分析英国与印度收集的全裂解血（Whole Lysed Blood, WLB）样本中，潜伏性结核、肺外结核、肺结核组及阴性对照样本的生物标志物蛋白表达水平。