MeDIP sequencing for human samples. MeDIP sequencing for human samples

We report a high-throughput analysis of human breast cancer cells using methylated DNA immunoprecipitation-sequencing.in this study to reveal the difference in methylation between breast cancer drug-resistant cells and sensitive cells. A total of 55076 differentially methylated genes (DMGs) were detected, including 21061 hypermethylated DMGs and 34015 hypomethylated DMGs. Moreover, Gene Ontology (GO) analysis and KEGG pathway analysis reveal the function and pathway of screening genes. These results indicate that DNA methylation may be involved in regulating the occurrence and development of breast cancer. Overall design: Examination of the whole-gene DNA methylation profile in breast cancer drug-resistant cells MCF-7 / Taxol and sensitive cells MCF-7

本研究采用甲基化DNA免疫沉淀测序（methylated DNA immunoprecipitation-sequencing）对人乳腺癌细胞开展高通量分析，旨在揭示乳腺癌耐药细胞与敏感细胞之间的甲基化差异。本研究共检测到55076个差异甲基化基因（differentially methylated genes，DMGs），其中高甲基化DMGs 21061个，低甲基化DMGs 34015个。此外，通过基因本体论（Gene Ontology，GO）分析及京都基因与基因组百科全书（Kyoto Encyclopedia of Genes and Genomes，KEGG）通路分析，本研究阐释了筛选得到的基因的功能及所参与的信号通路。上述结果表明，DNA甲基化可能参与调控乳腺癌的发生与发展进程。实验设计：检测乳腺癌耐药细胞MCF-7/Taxol与敏感细胞MCF-7的全基因组DNA甲基化谱。