MOESM2 of An engineered cryptic Hxt11 sugar transporter facilitates glucoseâ xylose co-consumption in Saccharomyces cerevisiae

Additional file 2. Supplementary tables. Table S1. SNPs identified in the DS68625-evo strain by genome sequencing. Table S2. Fermentation profiles by the selected S. cerevisiae strain expressing Hxt11 and the Hxt11 N366M and N366T mutants during anaerobic batch cultivation. Table S3. Oligonucleotides used in hexose transporter strain construction. Table S4. Oligonucleotides used for construction hexokinase deletion strain. Table S5. Oligonucleotides used in qPCR. Table S6. Primers used for saturation mutagenesis of HXT11.

附加文件2：补充表格。表S1：通过基因组测序在DS68625-evo菌株中鉴定得到的单核苷酸多态性（Single Nucleotide Polymorphisms, SNPs）。表S2：表达Hxt11以及Hxt11 N366M、N366T突变体的经筛选酿酒酵母（Saccharomyces cerevisiae, S. cerevisiae）菌株在厌氧分批培养过程中的发酵特性。表S3：己糖转运蛋白菌株构建所用的寡核苷酸。表S4：己糖激酶缺失菌株构建所用的寡核苷酸。表S5：实时定量聚合酶链反应（quantitative real-time polymerase chain reaction, qPCR）所用的寡核苷酸。表S6：HXT11饱和诱变所用的引物。