RNA-Seq analysis of human lung fibroblasts exposed to TGF-β

To assess the transcriptomic response of human lung fibroblast to TGF-β, human MRC5 pulmonary fibroblasts (CCL-171) were exposed or not to TGF-β at 10 ng/mL. RNA samples were harvested 24 hours after TGF-β exposition. Three independent experiments were carried out. 6 samples in triplicates: control versus TGF-β.为探究人类肺成纤维细胞对转化生长因子-β（TGF-β）的转录组应答反应，本研究将人MRC5肺成纤维细胞（CCL-171）以10 ng/mL浓度的TGF-β进行处理，同时设置未处理对照组。于TGF-β处理24小时后收集RNA样本。本研究共开展三次独立实验，实验设置对照组与TGF-β处理组，每组设置3次生物学重复，总计6个样本。