Supplemental data for 'Biophysical analysis reveals autophosphorylation as an important negative regulator of LRRK2 dimerization''

<strong>Supplemental mass photomerty raw data used in Figure 3</strong> of 'Guaitoli, G., Zhang, X., Saitta, F., Miglionico, P., Silbermann, L.M., Ho, F.Y., Zweydorf, F.v., Signorelli, M., Tych, K., Fessas, D., Raimondi, F., Kortholt, A., and Gloeckner, C.J. (2023). Biophysical analysis reveals autophosphorylation as an important negative regulator of LRRK2 dimerization. <em>bioRxiv</em>, 2023.2008.2011.549911 doi: 10.1101/2023.08.11.549911' <strong>Raw data Figure 3A:</strong> Mass photometry raw data for LRRK2 wild-type in presence of different G-nucleotides at different LRRK2 concentrations. Three biological replicates have been considered for the statistical analysis. <strong>Raw data Figure 3B: </strong>Mass photometry raw data for LRRK2 WT (left panel), LRRK2 WT + MLi-2 (middle panel) and kinase-dead LRRK2 (right panel) without and with ATP pre-incubation (-/+ ATP). Two biological and three technical replicates have been considered for the statistical analysis. <strong>Raw data Figure 3C</strong><strong>:</strong> Mass photometry raw data for pathogenic LRRK2 variants in presence of different G-nucleotides. Two biological and two technical replicates have been considered for the statistical analysis<strong>.</strong>**本数据集为下述论文中图3所用的补充性质量光度法（mass photometry）原始数据**：Guaitoli, G.、Zhang, X.、Saitta, F.、Miglionico, P.、Silbermann, L.M.、Ho, F.Y.、Zweydorf, F.v.、Signorelli, M.、Tych, K.、Fessas, D.、Raimondi, F.、Kortholt, A.与Gloeckner, C.J.（2023）。《生物物理分析揭示自磷酸化作为富亮氨酸重复激酶2（LRRK2）二聚化的重要负调控因子》，bioRxiv，2023.2008.2011.549911，DOI: 10.1101/2023.08.11.549911。 **图3A原始数据**：针对不同浓度的野生型LRRK2、添加不同G核苷酸时的质量光度法原始数据，统计分析采用三次生物学重复。 **图3B原始数据**：分别包含野生型LRRK2（左面板）、野生型LRRK2+MLi-2（中面板）与激酶失活型LRRK2（右面板）在未经与经三磷酸腺苷（ATP）预孵育（-/+ ATP）条件下的质量光度法原始数据，统计分析采用两次生物学重复与三次技术重复。 **图3C原始数据**：针对不同致病性LRRK2突变体、添加不同G核苷酸时的质量光度法原始数据，统计分析采用两次生物学重复与两次技术重复。