Rapid and Label-Free Structural Proteomics by One-Step Swift Trypsin LiP-MS
收藏NIAID Data Ecosystem2026-05-10 收录
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https://www.omicsdi.org/dataset/jpost/PXD069771
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资源简介：
We developed a rapid, surface-targeted limited proteolysis workflow, Swift Trypsin LiP-MS (STLiP-MS), to profile proteome-wide structural changes under near-native conditions.
STLiP-MS uses a trypsin-immobilized spin column and centrifugation-controlled contact to achieve instantaneous, highly reproducible limited digestion, followed by reduction/alkylation and desalting.
Proof-of-concept analyses were performed on HEK293T cell lysates (± phosphatase inhibitor PhosSTOP) and on a purified GPCR complex (A2A-BRIL with IgG).
Peptides were separated on a 75-µm ID C18 column (50 °C) using an UltiMate 3000 RSLCnano and analyzed on an Orbitrap HF-X.
Both DDA (top-50 HCD, 70-min gradient) and DIA (variable windows, 70-min gradient) runs were acquired.
DIA data were processed with DIA-NN (library-free/in-silico; UniProt human, 2024-03), and DDA of purified samples with PEAKS Studio 12.5.
创建时间：
2026-01-05
