Noggin vs BMP4 overexpression Epidermis. Mus musculus

The study was performed to determine if there were alterations in the total RNA pool among the epidermal keratinocytes of K14 promotor-driven noggin overexpression compared with K14 promotor-driven BMP4 overexpression transgenic animals, which will directly relate to cellular chemistry and immune and sensory function. The total study is also aimed at determining alterations of transcrption factors and/or regulation of gene function, including methylation states and micro RNA control in keratinocytes following sensory challenge, particularly neuropathic and chronic pain conditions. Keywords: Comparison of RNA expression between BMP4-oex and Nog-oex transgenic epidermal keratinocytes Overall design: Eight-ten epidermis strips were laser captured per animal, and six (6) animals were used per transgenic condition. Recovered RNA samples were pooled from 2 groups of 3 noggin transgenics and 2 groups of 3 BMP4 transgnics to create four pools for hybridization.本研究旨在探究K14启动子驱动的骨形态发生蛋白4（Bone Morphogenetic Protein 4, BMP4）过表达与K14启动子驱动的头蛋白（noggin）过表达的转基因动物的表皮角质形成细胞中总RNA库的差异，该差异与细胞化学、免疫及感觉功能直接相关。本研究同时旨在明确感觉刺激（尤其是神经病理性疼痛与慢性疼痛状态）下角质形成细胞内转录因子的变化、基因功能调控情况，包括甲基化状态与微小RNA（microRNA）的调控作用。关键词：BMP4过表达（BMP4-oex）与头蛋白过表达（Nog-oex）转基因表皮角质形成细胞的RNA表达对比。总体实验设计：每种转基因条件使用6只实验动物，每只动物获取8-10条激光捕获的表皮组织条带。将3只头蛋白转基因动物的RNA样本合并为2组，3只BMP4转基因动物的RNA样本合并为2组，最终获得4个混合RNA样本用于杂交实验。