Marchantia abiotic stress combination and diurnal gene expression atlas. Marchantia abiotic stress combination and diurnal gene expression atlas

In this study, we set to take advantage of Marchantias less complex signalling architecture to better understand how plants respond to environmental cues such as stress and time of the day, to modulate the expression of genes and biological pathways. To this end, we constructed an abiotic stress gene expression atlas of Marchantia comprising seven abiotic stresses (darkness, high light, cold, heat, nitrogen deficiency, salt, mannitol) and their pairwise combinations (e.g., cold + salt). We also measured gene expression at six timepoints of a day (12h light/ 12h darkness)

本研究旨在利用地钱（Marchantia）较为简单的信号传导架构，深入解析植物如何响应胁迫与昼夜时段等环境信号，进而调控基因表达与生物通路。为此，我们构建了地钱的非生物胁迫基因表达图谱，涵盖7种非生物胁迫：黑暗、高光、低温、高温、缺氮、盐胁迫、甘露醇胁迫，及其两两组合（如低温+盐胁迫）。此外，我们还测定了12小时光照/12小时黑暗的昼夜周期下6个时间点的基因表达水平。