Expression data from adult liver of offspring derived from cultured spermatogonia (GS cells) cryopreserved for a 6 months on the International Space Station (ISS) or on Earth.

Spermatogonial stem cells (SSCs) undergo self-renewal division to maintain spermatogenesis. In this study, we used SSCs for long-term cryopreservation in space flight. We derived germline stem cell (GS) cultures from DBA/2 mouse testes (Kanatsu-Shinohara et al., 2003, Biol. Reprod. 69, 216-616). GS cells were cryopreserved in the ISS for 6 months. Frozen GS cells were thawed upon return to Earth and tranplanted into the seminifelous tubules of infertile W mice. Recipients were housed with wild-type C57BL/6 females, and livers of F1 offspring were analyzed for gene expression. In this dataset, we include the expression data obtained from livers of 10 week old offspring derived from GS cells cryopreserved on the ISS or on Earth. As a control, we also include the expression data from the livers of wild-type BDF1 control mice. Each group contains 6 biological replicates.The liver of adult offspring were homogenised, and total RNA samples were extracted with TRIzol reagent (Invitrogen) and purified using the RNeasy cleanup system (QIAGEN, Valencia, CA). The RNAseq libraries were generated using the Illumina Stranded mRNA Prep Kit (Illumina, San Diego, CA). Sequencing was performed on an Illumina NextSeq 2000 platform with the paired-end mode.

精原干细胞（Spermatogonial stem cells, SSCs）可通过自我更新分裂维持精子发生过程。本研究将精原干细胞（SSCs）用于太空环境中的长期冷冻保存。我们从DBA/2小鼠睾丸中建立了生殖系干细胞（germline stem cell, GS）培养体系（Kanatsu-Shinohara等，2003，《生殖生物学》（Biol. Reprod.），69卷，216-616页）。将GS细胞置于国际空间站（International Space Station, ISS）中进行为期6个月的冷冻保存。返回地球后将冷冻保存的GS细胞复苏，并移植至不育W小鼠的生精小管中。将受体小鼠与野生型C57BL/6雌鼠合笼饲养，随后对F1代子代的肝脏进行基因表达分析。本数据集包含两类子代的肝脏表达数据：一类源自经国际空间站或地面冷冻保存的GS细胞所产生的10周龄子代。作为对照，本数据集同时纳入野生型BDF1对照小鼠的肝脏表达数据。每组设置6个生物学重复。将成年子代肝脏组织匀浆后，采用TRIzol试剂（Invitrogen公司）提取总RNA，并通过RNeasy纯化系统（QIAGEN，美国加利福尼亚州瓦伦西亚）进行纯化。采用Illumina链特异性mRNA建库试剂盒（Illumina，美国加利福尼亚州圣迭戈）构建RNA测序文库，并在Illumina NextSeq 2000平台上以双端测序模式完成测序。