Expression data changes induced by one round migration through 3 μm pores in T-ALL cells. Expression data changes induced by one round migration through 3 μm pores in T-ALL cells

One round migration through 3 μm pores promotes changes in transcription of T-ALL cells Overall design: Jurkat cells were maintained in culture in RPMI 1640 medium with L-glutamine and 125 mM HEPES (Sigma Aldrich, St. Louis, MO, USA) and 10% fetal bovine serum, FBS (Sigma-Aldrich) and maintained in 5% CO2 and 37ºC. Jurkat cells were allowed to migrate across 3 μm transwell inserts for 24h. Control (non-migrated cells) and one-round migration (ORM) cells were collected from the bottom chamber of Transwell inserts (Corning Costar, 6.5 mm diameter, 3 μm pore size) after 24h. Cells were lysed and mRNA was isolated and hybridized on Affymetrix microarrays to compare expression profiles. We used 2 replicates of each sample.

单次穿过3 μm孔径的迁移可诱导T细胞急性淋巴细胞白血病（T-ALL）细胞的转录变化。实验设计概述：Jurkat细胞采用添加L-谷氨酰胺、125 mM HEPES（美国密苏里州圣路易斯市Sigma Aldrich公司）、10%胎牛血清（Fetal Bovine Serum, FBS，Sigma-Aldrich）的RPMI 1640培养基进行体外培养，培养条件为37℃、5% CO₂环境。将Jurkat细胞置于孔径为3 μm的Transwell插入膜中迁移24小时。24小时后，分别从Transwell插入膜（Corning Costar，直径6.5 mm，孔径3 μm）的下室收集对照组（未迁移细胞）与单次迁移组（one-round migration, ORM）细胞。收集细胞并裂解，提取mRNA后进行Affymetrix基因芯片杂交以比较两组细胞的基因表达谱，每组样本设置2次重复。