Light chain biases and kinetics following influenza virus infections in ferrets

The kinetic profile of the hemagglutinin-specific antibody responses were tracked for 72 days after a primary infection with an influenza A or B virus and 14 days after a secondary infection with H1N1 influenza A virus. The HA binding of the serum antibodies and the number of HA-specific antibody-secreting cells were tested at multiple timepoints post-infection. The HA-specific immunoglobulin lambda light chain and kappa light chain frequencies were observed. Specific dominant anti-HA Ig light chain frequencies expressed following influenza A primary versus secondary virus infection that result in specific antibody activity.

本研究对甲型流感病毒（Influenza A）或乙型流感病毒（Influenza B）初次感染后72天内，以及甲型H1N1流感病毒二次感染后14天内的血凝素（hemagglutinin，HA）特异性抗体应答动力学特征进行了追踪。在感染后的多个时间点，研究人员检测了血清抗体的HA结合活性与HA特异性抗体分泌细胞的数量，并观测了HA特异性免疫球蛋白λ轻链与κ轻链的占比频率；同时分析了甲型流感病毒初次与二次感染后，表达出的可介导特异性抗体活性的优势抗HA免疫球蛋白轻链的占比情况。