Transcriptome Analysis for Antagonistic Molecular Mechanism of Isolated Probiotic Bacteria against Multi Drug Resistant E. coli.. Bacteria

We isolated probiotic bacterial strain TY-11 from yogurts and conducted Transmission Electron Microscope (TEM) experiment, whole genome sequencing, functional and comparative genomic study, biochemical tests, as well as mRNA analysis. From the complete genome sequence and phylogenetic tree constructed with 332 core genes from 262 genomes, the isolate TY-11 was identified as Lactobacillus delbrueckii subsp. indicus. This probiotic killed the multi-drug resistant (MDR) Escherichia coli JW3481-KC within 4 hours, although any kind of distortion was not observed in the surface of the cells of Escherichia coli JW3481-KC under TEM. From this proposed study, we got the insights of potential probiotic features and antimicrobial activities of our isolated probiotic TY-11, which killed Escherichia coli JW3481-KC by secreting acids and hydrogen peroxide in co-culture.