Gene expression of sorted Eomes+ and Eomes‒ γδ T cells from spleen of Eomes-IRES-GFP mice.

Eomesodermin (Eomes) is a transcription factor with a crucial role regulating cytotoxic function, development and survival of immune cells. Although it is known that γδ T cells can express Eomes, its function on those cells is still largely unknown. Using Eomes-IRES-GFP mice we were able to sort for Eomes+ and Eomes‒ γδ T cells populations and get their gene expression profiles, bringing light to the role of Eomes on γδ T cells. Eomes+ and Eomes‒ γδ T cells samples were obtained from three completely independent flow cytometric sorts. As a source, cells from spleen from 4 to 6 Eomes-IRES-GFP were prepared for each sort.

Eomesodermin (Eomes)是一种转录因子，在调控免疫细胞的细胞毒性功能、发育与存活过程中发挥关键作用。尽管已知γδ T细胞（γδ T cells）可表达Eomes，但其在该类细胞中的功能仍未完全明确。本研究借助Eomes-IRES-GFP小鼠，成功分选出Eomes阳性（Eomes+）与Eomes阴性（Eomes‒）的γδ T细胞群体，并获取了其基因表达谱，从而阐明了Eomes在γδ T细胞中的功能。Eomes阳性与Eomes阴性的γδ T细胞样本均取自3次完全独立的流式细胞分选。每次分选的实验材料均来自4~6只Eomes-IRES-GFP小鼠的脾脏细胞。