Transcriptional profiling of putative ABC Transporter gene orf19.4531 knockout in Candida albicans

Since, ABC transporters do perform various physiological roles.To get better insight about possible involvement of putative ABC transporter orf19.4531 in different functions transcriptional profiling have been peformed and compared between WT(SC5314) Candida albicans cells vs putative orf19.4531 knockout cells after 6hrs growth of culture.The assay as peformed in biological duplicate sample. Agilent one-color experiment,Organism: Candida albicans ,Custom Agilent Candida albicans 8x15k Microarray Gene expression (AMADID: 026377) designed by Genotypic Technology Private Limited , Labeling kit: Agilent Quick-Amp labeling Kit (p/n5190-0442)

鉴于ATP结合盒（ABC transporter）转运蛋白承担着多种生理功能。为深入探究推定的ABC转运蛋白orf19.4531可能参与的各类生物学功能，本研究对培养6小时后的野生型（WT，SC5314）白色念珠菌（Candida albicans）细胞与orf19.4531基因敲除细胞进行转录谱分析，并对比两组样本的分析结果。本实验设置生物学重复，每组包含两个重复样本。实验采用安捷伦单通道芯片平台，实验生物为白色念珠菌；定制化安捷伦白色念珠菌8x15k基因表达微阵列（AMADID: 026377）由Genotypic Technology Private Limited公司设计；标记试剂盒选用安捷伦Quick-Amp标记试剂盒（货号：p/n5190-0442）。