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Z. Xu, H. Ogawa, P. Vagnarelli, J.H. Bergmann, D.F. Hudson, S. Ruchard, T. Fukagawa, W.C. Earnshaw, K. Samejima (2011) CIL:12165, Gallus gallus gallus, memory B cell. CIL. Dataset

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Cell of the DT40 chicken B lymphocyte line at mitosis. Z-series of widefield immunofluorescence images captured using a 100x/1.4NA planapo objective lens on a Deltavision system and deconvolved with a constrained iterative algorithm (Applied Precision). Displayed is the central z-slice. Metaphase cell localization of exogenous TrAP-INCENP (red) plus endogenous Aurora B (green) and DNA (blue) in INCENPOFF cells expressing TrAP-INCENP WT. See Fig 6A panel 1 in Xu et al. 2009 J Cell Biol 187, 637-653.

DT40鸡B淋巴细胞系细胞在有丝分裂阶段的细胞核切片。利用Deltavision系统配备的100倍/1.4NA Planapo物镜拍摄的宽场免疫荧光图像的Z系列,经约束迭代算法(Applied Precision)去卷积处理。展示的是中心Z切片。在表达TrAP-INCENP WT的INCENPOFF细胞中,观察外源TrAP-INCENP(红色)与内源Aurora B(绿色)及DNA(蓝色)的中期细胞定位。详见Xu等人在2009年《细胞生物学杂志》(J Cell Biol)187卷637-653页的第6A图面板1。
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