Secondary structure and backbone relaxation analysis of MPS-1(134–256) in the absence or presence of DPC micelles.

(A) TALOS + secondary structure prediction were based on the assigned backbone 13CO, 13Cα, 13Cβ chemical shifts of MPS-1(134–256) in the presence or absence of DPC. Only random coil secondary structure was observed for MPS-1(134–256) in both aqueous buffer and DPC micelles. (See Fig. S3 in file S1 for the Y-axis scale from 0 to 0.3) (B) Distribution of the measured 15N T1 longitudinal and T2 transverse relaxation times and steady-state 1H-15N NOE values along the primary sequence in the absence (black) or presence (red) of DPC micelles.

(A) TALOS与二级结构预测基于已指认的MPS-1(134–256)在有或无十二烷基磷酸胆碱（DPC）存在时的主链13CO、13Cα、13Cβ化学位移。在水溶液缓冲液与DPC胶束两种体系中，MPS-1(134–256)均仅呈现无规卷曲二级结构（详见补充材料文件S1中的图S3，其纵轴刻度范围为0至0.3）。
(B) 无（黑色曲线）与有（红色曲线）DPC胶束存在时，沿蛋白一级序列分布的实测15N纵向弛豫时间T1、横向弛豫时间T2，以及稳态1H-15N核奥弗豪泽增强（NOE）值的分布情况。