Comparisons of automated Leica <i>HER2</i> FISH with digital analysis and manual Abbott <i>HER2</i> FISH with manual analysis of 50 invasive breast cancer tissue specimens (resection / biopsy) with IHC 2+ scores.

FISH, fluorescence in situ hybridization. a ratio ≥ 2.2. b ratio = 1.8–2.2. c ratio <1.8. d 3 categories (amplified / equivocal / non-amplified). e 2x) reanalysis Leica FISH confirms amplification Abbott FISH; 1x) new staining Abbott FISH confirms non-amplified score Leica FISH (first Abbott FISH contained 3 biopsies, whereas the biopsy in the middle is missing in the new Abbott FISH and Leica FISH). 2x missing data because no signals were detected with Leica FISH. Comparisons of automated Leica HER2 FISH with digital analysis and manual Abbott HER2 FISH with manual analysis of 50 invasive breast cancer tissue specimens (resection / biopsy) with IHC 2+ scores.FISH，即荧光原位杂交（fluorescence in situ hybridization）。 a 比值≥2.2。 b 比值为1.8~2.2。 c 比值<1.8。 d 分为3个类别：扩增/可疑/未扩增。 e 2例：经重新分析的徕卡（Leica）FISH结果证实，雅培（Abbott）FISH判定为扩增；1例：新染色的雅培FISH结果确认，徕卡FISH评分为未扩增（首次雅培FISH包含3份活检样本，而新的雅培FISH与徕卡FISH中缺失了中间的那份活检样本）。另有2例缺失数据，原因是徕卡FISH未检测到信号。 本数据集针对50例IHC（免疫组化，Immunohistochemistry）评分为2+的浸润性乳腺癌组织样本（切除标本/活检标本），分别采用自动化徕卡HER2 FISH结合数字化分析、手动雅培HER2 FISH结合人工分析进行对比检测。