Expression data from control and Snai1 knockout embryonic stem cells (ESCs). Mus musculus

Snail1 is a master epithelial-mesenchymal trisition (EMT) factor but its role in ESC maintenance is unknown. We used microarrays to compare the global gene expression between control and Snai1 knockout ESCs. Overall design: RNA extracted from control and Snai1 knockout ESCs were hybridizated on Affymetrix microarrays.

Snail1是核心上皮-间质转化（epithelial-mesenchymal transition, EMT）调控因子，但其在胚胎干细胞（Embryonic Stem Cell, ESC）维持中的作用尚未明确。本研究采用基因芯片技术，对比了对照组与Snail1敲除胚胎干细胞的全基因表达谱。实验整体设计：从对照组及Snail1敲除的胚胎干细胞中提取的RNA，均在Affymetrix基因芯片上完成杂交检测。