Bulk RNA-seq on mouse model of diabetic nephropathy and in vitro model of SRSF7 knockdown

In this dataset, we utilized the db/db, uninephrectomy and renin-hypertension mouse model. We performed bulk RNA-seq and compared vehicle to ACE inhibitor, Rosiglitizone, SGLT2 inhibitor, ACEi + Rosiglitizone and ACEi + SGLT2i at two time points (2 days and 2 weeks). To study the mechanism, we also performed bulk RNA-seq on human primary tubular epithelial cells with or without SRSF7 siRNA knockdown. Overall design: We performed bulk RNA-seq to a model of murine DKD and in vitro model of SRSF7 siRNA knockdown.

本数据集采用了db/db小鼠、单侧肾切除术小鼠及肾素高血压小鼠模型。我们开展了批量RNA测序（bulk RNA-seq），并在2天、2周两个时间点下，对载体对照组、血管紧张素转换酶抑制剂（ACE inhibitor）、罗格列酮、钠-葡萄糖协同转运蛋白2抑制剂（SGLT2 inhibitor）、ACEI联合罗格列酮以及ACEI联合SGLT2抑制剂各处理组进行了对比分析。为探究相关作用机制，我们还对转染或未转染SRSF7小干扰RNA（small interfering RNA，siRNA）进行敲低的人原代肾小管上皮细胞开展了批量RNA测序。整体实验设计：本研究针对小鼠糖尿病肾病（diabetic kidney disease, DKD）模型以及SRSF7基因siRNA敲低的体外模型开展了批量RNA测序。