Chromatin-seq of Arabidopsis thaliana

We present chromatin structure maps of the A. thaliana Col-0 wild-type (in vitro cell culture) combining differential micrococcal nuclease (MNase) digestion and analysis by paired-end next-generation sequencing to provide insight into the sensitive genomic regions and variable DNA-bound particle size. The landscape of sub-nucleosomal sized particles (subNSPs) has been investigated, revealing the DNA-binding complex positioning genome-wide. We observe the dynamic changes in the distribution of these complexes surrounding genomic features本研究构建了拟南芥（A. thaliana）Col-0野生型（体外细胞培养）的染色质结构图谱，通过结合差分微球菌核酸酶（micrococcal nuclease，MNase）消化法与配对端下一代测序分析，为解析敏感基因组区域及可变DNA结合颗粒尺寸提供了研究视角。本研究针对核小体亚尺寸颗粒（subNSPs）的分布特征展开探究，揭示了全基因组范围内的DNA结合复合物定位规律。本研究观测到这些复合物在基因组特征周边的分布存在动态变化。