Effects of lipid loading on splenic cDC2s. Effects of lipid loading on splenic cDC2s

To investigate the effect of lipid loading on DCs, cDC2 are isolated from the spleen from mice that are fed a Western-type diet and RNAseq was utilized to analyze the gene expression Overall design: Gene expression profiling using RNAseq为探究脂质负载对树突状细胞（Dendritic Cells, DCs）的影响，本研究从喂食西式饮食（Western-type diet）的小鼠脾脏中分离经典树突状细胞2型（classical dendritic cell 2, cDC2），并采用RNA测序（RNA sequencing, RNAseq）分析其基因表达。实验整体设计：采用RNAseq开展基因表达谱分析。