HPRC samples R9.4.1 bam files (27)

Raw basecalled BAM files were downloaded from human pangenome reference consortium https://s3-us-west-2.amazonaws.com/human-pangenomics/index.html?prefix=working/.BAM files were re-aligned to the GRCh38 reference genome (GenBank assembly ID GCA_000001405.15) using Dorado v0.7.2 with command "dorado aligner -t 80 --mm2-preset "-x map-ont" ${reference.fa} ${input.bam} --output-dir ${outdir}/${output}", followed by catting, sorting , and indexing with Samtools.

我们从人类泛基因组参考联盟（Human Pangenome Reference Consortium）的公开资源地址https://s3-us-west-2.amazonaws.com/human-pangenomics/index.html?prefix=working/ 下载了原始碱基识别后的BAM文件（BAM）。随后使用Dorado v0.7.2工具，以命令"dorado aligner -t 80 --mm2-preset "-x map-ont" ${reference.fa} ${input.bam} --output-dir ${outdir}/${output}"，将这批BAM文件重新比对至GRCh38参考基因组（基因库（GenBank）组装编号GCA_000001405.15）；比对完成后，通过Samtools工具完成文件合并、排序与索引构建操作。