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Mosquitoes are the primary vectors of arthropod-borne pathogens. Aedes aegypti is one of the most widespread mosquito species worldwide, responsible for transmitting diseases such as Dengue, Zika, and Chikungunya, among other medically significant viruses. Characterizing the array of viruses circulating in mosquitoes, particularly in Aedes aegypti, is a crucial tool for detecting and developing novel strategies to prevent arbovirus outbreaks. In this study, we address the implementation of a sequencing and analysis pipeline based on the Oxford Nanopore Technologies MinION Mk1b system, for arboviral detection in field-caught mosquitoes from Argentina. Full genome of Humaita Tubiacanga Virus (HTV), Phasi Charoen-like Phasivirus (PCLV), Aedes aegypti totivirus (AaeTV) has been sequenced in three distinct regions of Argentina comprising Buenos Aires province, Santa Fe province and the northern province of Salta. Viral sequences enriched by SISPA and coupled with Nanopore sequencing can be a useful tool for viral surveillance, not only for detecting viruses that have a high impact on human and animal health, but also for detecting insect-specific viruses that could promote the transmission of arboviruses.蚊虫是节肢动物传播病原体的主要媒介。埃及伊蚊（Aedes aegypti）是全球分布最广泛的蚊虫种类之一，可传播登革热、寨卡病毒、基孔肯雅热等多种具有医学重要性的病毒。对蚊虫（尤其是埃及伊蚊）体内循环的各类病毒进行特征解析，是检测并开发新型虫媒病毒暴发防控策略的关键手段。本研究搭建了基于牛津纳米孔科技公司（Oxford Nanopore Technologies）MinION Mk1b系统的测序与分析流程，用于检测阿根廷野外捕获蚊虫中的虫媒病毒。研究团队已在阿根廷布宜诺斯艾利斯省、圣菲省以及北部的萨尔塔省三个不同区域，完成了乌马伊图亚坎加病毒（Humaita Tubiacanga Virus, HTV）、披色查仑样杆状病毒（Phasi Charoen-like Phasivirus, PCLV）以及埃及伊蚊托蒂病毒（Aedes aegypti totivirus, AaeTV）的全基因组测序。通过序列无关单引物扩增（Sequence-independent single primer amplification, SISPA）富集病毒序列并结合纳米孔测序技术，可作为病毒监测的有效工具：不仅可用于检测对人类与动物健康具有重大影响的病毒，还可识别可能促进虫媒病毒传播的昆虫特异性病毒。