Wetland soil Raw sequence reads

Underground injection of CO2 is a frequently discussed option to translocate anthropogenic CO2 emissions, but the environmental consequences of leakages are poorly understood. As a natural analogue, we studied a floodplain wetland area where localized long-term emanations of cold volcanic CO2 (mofettes) altered soil formation and led to stable hypoxia, as well as increased soil C storage (up to 47.3 w.-% C). We hypothesized that CO2 degassing is associated with functional changes in the mofette soil biome, resulting in increased C input and/or impeded mineralization. With a multi-pronged approach, involving depth-resolved C isotope geochemistry, soil activity, metatranscriptome and metagenome analysis, we identified significant differences in mofette C and energy flow compared to an unaffected floodplain wetland soil.地下注入二氧化碳（CO₂）是学界广泛讨论的一种人为碳排放转移方案，但其泄漏引发的环境后果尚未得到充分认知。作为该技术场景的自然类比研究对象，我们针对一处洪泛平原湿地展开调研：该区域存在局部长期释放的冷火山二氧化碳喷溢点（mofettes），其改变了当地土壤的形成过程，造就了持续稳定的缺氧环境，并使土壤碳储量提升至47.3重量百分比的碳。我们提出研究假说：二氧化碳脱气过程与该喷溢点土壤生物群落的功能改变存在关联，进而导致碳输入增加或矿化作用受阻（或二者兼具）。本研究采用多维度研究手段，涵盖深度分层碳同位素地球化学分析、土壤活性检测、宏转录组学（metatranscriptome）与宏基因组学（metagenome）分析等，结果发现相较于未受干扰的洪泛平原湿地土壤，该二氧化碳喷溢点区域的碳循环与能量流动存在显著差异。