The Inhibitory Concentration (IC₅₀) required to reduce number of bsf trypomastigotes by 50%.

Pluronic were tested at 12 serial dilutions in triplicate and repeated in 3 separate experiments (n = 3) to produce IC50 values.普朗尼克（Pluronic）共设置12个系列稀释梯度，每个梯度均完成三次平行检测，并在3次独立实验中重复该实验操作（n=3），最终获得半数抑制浓度（IC50）值。