Transcriptome analysis of hepatocellular carcinoma and adjacent non-tumor liver tissues [I]. Transcriptome analysis of hepatocellular carcinoma and adjacent non-tumor liver tissues [I]

Integrin alpha 6 (ITGA6) forms integrin receptors with either integrin β1 (ITGB1) or integrin beta 4 (ITGB4). To study its expression and regulatory functions in hepatocellular carcinoma (HCC) progression, whole transcriptome RNA sequencing in paired HCC tumor and adjacent non-tumor liver tissue samples from ten local Hispanic patients was performed. The average expression of ITGA6 was found increased by over 3.5-fold in HCC tumor tissues in comparison with their adjacent non-tumor tissues. The study indicates integrin alpha6-beta4 complex as a potential therapeutic target for treating patients in HCC. Overall design: 10 tumor-normal pairs of HCC samples were profiled using high-throughput RNA sequencing techniques and their relative gene expression levels were compared.

整合素α6（Integrin alpha 6, ITGA6）可分别与整合素β1（integrin β1, ITGB1）或整合素β4（integrin beta4, ITGB4）结合形成整合素受体。为探究其在肝细胞癌（hepatocellular carcinoma, HCC）进展过程中的表达模式与调控功能，本研究对10名当地西班牙裔患者的配对肝细胞癌肿瘤组织及癌旁正常肝组织样本开展了全转录组RNA测序。结果显示，相较于配对癌旁正常组织，肝细胞癌肿瘤组织中ITGA6的平均表达量上调超过3.5倍。本研究提示整合素α6-β4复合物可作为肝细胞癌患者的潜在治疗靶点。整体实验设计：采用高通量RNA测序技术对10例肝细胞癌的肿瘤-正常组织配对样本进行测序分析，并比较其相对基因表达水平。