miRNA-seq of lung in two kinds of pig breeds after PCV2 injection. Sus scrofa

Postweaning multisystemic wasting syndrome in pigs has devastated the swine industry since the 1990s. Porcine circovirus type 2 (PCV2) is the primary cause of this disease. MicroRNAs (miRNAs) are small noncoding RNAs that play important roles in regulating gene expression, especially at the post-transcription level. The expression profiles of miRNAs have been reported in porcine reproductive and respiratory syndrome, porcine parvovirus, and other pig diseases; however, the miRNA expression profiles in pigs infected with PCV2 have not been reported so far. The Laiwu pig (a Chinese indigenous pig breed) has different meat quality, adipogenesis, and disease-resistance from western commercial pig breeds. In this study, four small RNA libraries were constructed from the lung tissue of uninfected and infected Laiwu and Yorkshire/Landrace crossbred (YL) pigs. High-throughput sequencing and bioinformatics were used to determine the abundance and differential expression of miRNAs in the four libraries Overall design: miRNA-seq of four groups as PCV2 infected Laiwu pig (Lw-i), uninfected Laiwu pig (Lw-un), infected western commercial (YL-i) and uninfected western commercial pigs (YL-un)

自20世纪90年代起，猪断奶后多系统衰竭综合征（Postweaning multisystemic wasting syndrome）便对养猪业造成了严重破坏。猪圆环病毒2型（Porcine circovirus type 2, PCV2）是该疾病的主要致病原。微小RNA（microRNAs, miRNAs）是一类小型非编码RNA，在基因表达调控尤其是转录后水平发挥着关键作用。已有研究报道了猪繁殖与呼吸综合征、猪细小病毒及其他猪类疾病中的miRNA表达谱，但目前尚未见猪感染PCV2后的miRNA表达谱相关研究报道。莱芜猪作为中国本土猪品种，在肉质、脂肪生成能力与抗病性方面均与西方商业猪品种存在显著差异。本研究从未感染与感染PCV2的莱芜猪，以及约克夏/长白杂交（YL）猪的肺组织中构建了4个小RNA文库。采用高通量测序与生物信息学分析手段，对4个文库中miRNA的表达丰度与差异表达情况进行了检测。整体实验设计：对4组样本开展miRNA测序，分组分别为PCV2感染莱芜猪（Lw-i）、未感染莱芜猪（Lw-un）、感染西方商业猪（YL-i）以及未感染西方商业猪（YL-un）