Gene expression profile at single cell level for human metastatic melanoma samples

There exists great intra- and inter-tumor heterogeneity for metastatic melanomas. We used single cell RNA sequencing (scRNA-seq) to analyze cell type heterogeneity of metastatic melanoma and cell type specific responses to different treatments. Overall design: We enzymatically digested and dissociated fresh tumor samples and analyzed using scRNA-seq.转移性黑色素瘤存在显著的瘤内与瘤间异质性。本研究采用单细胞RNA测序（single cell RNA sequencing, scRNA-seq）技术，分析转移性黑色素瘤的细胞类型异质性，以及不同治疗方案下的细胞类型特异性应答反应。整体实验设计：我们通过酶解消化解离新鲜肿瘤样本，并采用scRNA-seq技术完成分析。