mRNA expression analysis of AR, HOXC6 and NKX2-2 overexpression

Investigation of mRNA expression level changes in RWPE1 cells with AR,HOXC6 and NKX2-2 overexpression. RNA quantity and quality were measured by NanoDrop ND-1000. RNA integrity was assessed by standard denaturing agarose gel electrophoresis. The Human 12 x 135K Gene Expression Array was manufactured by Roche NimbleGen. This array enables you to conveniently and simultaneously hybridize 12 samples on each slide. About 45033 genes were collected from the authoritative data source including NCBI.本研究旨在探究过表达AR、HOXC6及NKX2-2的RWPE1细胞内mRNA表达水平的变化情况。实验采用NanoDrop ND-1000仪器测定RNA的浓度与质量，并通过标准变性琼脂糖凝胶电泳评估RNA的完整性。本研究使用的人类12×135K基因表达芯片由罗氏尼姆博基因（Roche NimbleGen）制造，该芯片可在每张玻片上同时便捷地完成12个样本的杂交反应。该芯片共收录了来自包括美国国家生物技术信息中心（National Center for Biotechnology Information，NCBI）在内的权威数据源的约45033个基因。