RNA-seq analysis of TIGRa variants

RNA-seq analysis of HEK293T cells transfected with an HBB-targeting sgRNA (sgHBB) or a non-targeting control (sgNT) for three TIGRa variants and dCasMINI-VPR (positive control).针对三种TIGRa变体与作为阳性对照的dCasMINI-VPR，分别采用靶向HBB的单引导RNA（single-guide RNA，sgRNA）或非靶向对照单引导RNA（sgNT）转染HEK293T细胞，随后对这些细胞开展RNA测序（RNA-seq）分析。