<i>In vivo</i> activities and accumulation of LacI-GFP insertion proteins.

1Repression activity = β-gal activity of CSH140 divided that of the indicated plasmid in CSH140. Cells were grown in the absence of IPTG. At least three cultures were assayed. 2Inducibility = Repression activity of cells grown in the absence of IPTG divided by that of cells grown in the presence of IPTG. At least three cultures were assayed. 3Protein levels were determined by quantifying the gel shown in Figure 2B. Relative protein levels were calculated by dividing each sample by the protein level of LacI-99-GFPi, which had the lowest protein accumulation. 4NA = Not applicable.1. 阻遏活性 = CSH140的β-半乳糖苷酶（β-galactosidase, β-gal）活性除以CSH140中对应指示质粒的β-半乳糖苷酶活性。实验细胞在不含异丙基-β-D-硫代半乳糖苷（IPTG）的条件下培养，至少对3个独立培养物进行了检测。 2. 诱导性 = 不含IPTG培养的细胞的阻遏活性除以含IPTG培养的细胞的阻遏活性。至少对3个独立培养物进行了检测。 3. 蛋白水平通过对图2B所示的凝胶进行定量分析确定。相对蛋白水平通过将每个样品的蛋白水平除以蛋白积累量最低的LacI-99-GFPi的蛋白水平计算得到。 4. NA = 不适用（Not applicable）。