Genome-wide H3k27ac levels following FOXA1 KO in UMUC-1 human bladder cancer cells [scRNA-Seq]. Genome-wide H3k27ac levels following FOXA1 KO in UMUC-1 human bladder cancer cells [scRNA-Seq]

10x scRNA-Seq results of a bladder cancer case with extensive squamous cell differentation Overall design: Fresh tumor tissue were digesteThe tumor sample analyzed by single cell (sc)-RNASeq was dissociated (Miltenyi biotec) at 37 °C for 30 min. A single cell suspension was then analyzed using the 10x genomics platform.

一例伴广泛鳞状细胞分化的膀胱癌患者的10x单细胞RNA测序（10x scRNA-Seq）结果。整体实验设计：将新鲜肿瘤组织进行消化处理，随后采用美天旎（Miltenyi Biotec）的解离方法于37℃下孵育30分钟以制备单细胞悬液，最终通过10x Genomics平台完成测序分析。