Supporting data for “ESTROGEN ACTIVATION ON HISTONE H3 LYSINE 79 DIMETHYLATION MEDIATED MENIN AND CHROMATIN INTERACTION IN BREAST CANCER"

This dataset is my thesis.In order to understand the dynamics between epigenetic regulation and hormone receptor interactions, proteomic and genomic approaches will be used to explore the effects of oestrogen activation on H3K79me2-mediated Menin/chromatin interactions as well as the mechanism of Menin - DOT1L - ERalpha interactions with chromatin in breast cancer cells.We designed several experiments such as chromatin immunoprecipitation (ChIP).The dataset includes the results of qPCR analyses for ChIP, western blot data relevant to the project, and datasets from anti-Menin, anti-DOT1L and anti-ERalpha co - IP experiments resolved by mass spectrometry.本数据集为本人学位论文的研究数据。为阐明表观遗传调控与激素受体互作间的动态关联，本研究拟采用蛋白质组学与基因组学方法，探究雌激素激活对H3K79me2介导的Menin/染色质互作的影响，以及乳腺癌细胞内Menin-DOT1L-ERalpha与染色质互作的分子机制。本研究设计了包括染色质免疫共沉淀（chromatin immunoprecipitation, ChIP）在内的多项实验。本数据集包含ChIP实验的实时定量PCR（quantitative real-time PCR, qPCR）分析结果、与本项目相关的蛋白质免疫印迹（Western blot）数据，以及经质谱鉴定的抗Menin、抗DOT1L与抗ERalpha免疫共沉淀实验相关数据集。