Klf5 confers embryonic and extra-embryonic bi-potential cell fate in early blastomeres and embryonic stem cells. Klf5 confers embryonic and extra-embryonic bi-potential cell fate in early blastomeres and embryonic stem cells

This study determines the genome-wide binding profile of a V5-tagged KLF5 transription factor exogenously expressed in mouse embryonic stem cells. Overall design: V5 ChIP-seq was performed in wild type mouse ES cells overexpressing a V5-tagged KLF5 protein, and in wild-type D3 mouse ES cells as a control. Normal IgGs pulldowns and input DNA were also sequenced as controls.

本研究解析了外源表达V5标签化KLF5转录因子的小鼠胚胎干细胞中的全基因组结合图谱。实验设计概况：分别在过表达V5标签化KLF5蛋白的野生型小鼠胚胎干细胞，以及作为对照的野生型D3小鼠胚胎干细胞中开展V5染色质免疫沉淀测序（ChIP-seq）实验。同时还对正常IgG免疫沉淀下拉样本与输入DNA进行测序，作为对照。