Dependence of cell doubling time on generation number for old pole and new pole daughter cells.

a) Old pole daughter cells took longer to divide than new pole daughter cells (our linear mixed model analysis returned χ2df=1 =5.192, p = 0.023). For both cell types, division time progressively increased over successive generations (our linear mixed model analysis returned χ2df=1 = 38.327, p &lt; 0.001). This analysis was carried out including data for generations 2 to 11 from two experimental runs.<br>(b) Inspecting the 24-generation data set revealed that the progressive increase in division times gradually asymptotes to a steady state division time with increasing generation number (LMM: linear effect of generation number: χ2df=1 = 52.199, p &lt; 0.001; quadratic effect of generation number: χ2df=1 = 32.832, p &lt; 0.001). Old pole daughter cells also took longer to divide than new pole daughter cells within this 24-generation data set (LMM: χ2df=1 = 11.222, p = 0&lt;0.001). <br>

(a) 老极子代细胞的分裂时长显著长于新极子代细胞（本研究采用线性混合模型（linear mixed model）分析，得到自由度为1的卡方统计量χ²=5.192，p=0.023）。两类子代细胞的分裂时长均随传代次数的增加而逐步延长（线性混合模型分析结果显示，自由度为1的卡方统计量χ²=38.327，p<0.001）。本次分析纳入了两次独立实验中第2至11代的细胞数据。<br>(b) 对24代数据集的检视结果表明，分裂时长随代次增加的渐进式增长会逐渐趋于稳态分裂时长（线性混合模型（linear mixed model, LMM）分析：代次的线性效应：自由度为1的卡方统计量χ²=52.199，p<0.001；代次的二次效应：自由度为1的卡方统计量χ²=32.832，p<0.001）。在该24代数据集内，老极子代细胞的分裂时长同样显著长于新极子代细胞（线性混合模型分析：自由度为1的卡方统计量χ²=11.222，p<0.001）。