RNA-Seq analysis of WT and Phlpp1-deficient alveolar macrophages in bleomycin-induced PF model

The purpose of this study is to detect differentially expressed genes in wild-type (WT) and Phlpp1-deficient alveolar macrophages (AMs) 7 days after bleomycin. We performed gene expression profiling of AMs of broncho-alveolar lavage fluid (BALF) from mice that were intratracheally instilled with saline or bleomycin. Gene expression differences between the two samples could be found using transcriptome profiling (RNA-seq) analysis. RNA profiles were generated by deep sequencing, using Illumina. AMs were stained to confirm the surface expression of CD11c and Siglesc-F. Cells with purity >98% were used for subsequent experiments. We identified several differentially expressed genes in the two groups of cells. Overall design: Expression profiling by high throughput sequencing

本研究旨在检测博莱霉素处理7天后，野生型（wild-type，WT）与Phlpp1基因缺陷型肺泡巨噬细胞（alveolar macrophages, AMs）中的差异表达基因。我们对经气管内滴注生理盐水或博莱霉素的小鼠的支气管肺泡灌洗液（broncho-alveolar lavage fluid, BALF）中的肺泡巨噬细胞开展基因表达谱分析。借助转录组测序（RNA-seq）分析，可鉴定两组样本间的基因表达差异。本研究采用Illumina平台进行深度测序以获取RNA表达谱。我们对肺泡巨噬细胞进行染色，以验证CD11c与Siglesc-F的表面表达水平，选取纯度大于98%的细胞用于后续实验。我们在两组细胞中鉴定出若干差异表达基因。整体实验设计：基于高通量测序的表达谱分析