Repeat analysis of PBMC aliquots obtained from HIV-1 infected children.

For three HIV-1 infected children receiving Kaletra once daily, three aliquots of one million PBMC each were processed as described in the text (solid phase extraction followed by methanol extraction, and spotting in triplicate) to determine the overall precisions (%CV) of PBMC processing and sample measurements. Shown are the concentrations of lopinavir (LPV) and ritonavir (RTV) in µM as determined in a single spot on the target plate.

针对3名每日单次服用克力芝（Kaletra）的HIV-1感染儿童，取3份每份含100万外周血单个核细胞（PBMC）的等分试样，按照文中所述方法处理：先进行固相萃取（Solid Phase Extraction），再经甲醇萃取（Methanol Extraction），并进行一式三份点样，以测定PBMC处理与样本检测的总精密度（变异系数，%CV）。结果展示了靶板上单一点样中测得的洛匹那韦（LPV）与利托那韦（RTV）的浓度，单位为微摩尔（μM）。