Transcriptomic analysis of interleukin 22 deficient medaka against DSS exposure. Oryzias latipes strain:Cab

In this study, interleukin (IL)-22 knockout (KO) medaka was established using genome editing system, CRISPR-Cas9. Furthermore, to explore roles of IL-22 when experimental inflammation induced, larval KO medaka were exposured to Dextran sulfate sodium (DSS) containing water and RNA-seq was performed.

本研究利用CRISPR-Cas9基因组编辑系统，构建了白细胞介素（interleukin，IL）-22敲除（knockout，KO）青鳉模型。此外，为探究IL-22在实验性炎症诱导过程中的作用，本研究将IL-22敲除青鳉幼鱼暴露于含葡聚糖硫酸钠（DSS）的水环境中，并开展RNA测序（RNA-seq）分析。