Additional file 4: of Development of EMS-induced mutation population for amylose and resistant starch variation in bread wheat (Triticum aestivum) and identification of candidate genes responsible for amylose variation

Normalized threshold cycle (ΔCт) of chromosome specific alleles of GBSSI (GBSSI–7A, GBSSI–4A, and GBSSI–7D) during seed development. The data was taken at three stages of seed development (21, 28, and 35 days after anthesis, DAA) in two mutant lines, ‘TAC 75’(amylose content - 64 %) and ‘TAC 6’(amylose content– 6.8 %), in comparison to the parent variety, ‘C 306’ (amylose content - 26 %). The data was taken in two biological replications, each represented as mean ± SD from three technical replicates. Wheat ADP Ribosylation Factor (ARF) was used as an internal control gene for normalization. (XLSX 12 kb)本数据集包含小麦籽粒发育过程中颗粒结合淀粉合酶I（GBSSI）的染色体特异性等位基因（GBSSI–7A、GBSSI–4A及GBSSI–7D）的归一化循环阈值（ΔCт）。实验以两个突变株系‘TAC 75’（直链淀粉含量64%）与‘TAC 6’（直链淀粉含量6.8%）为研究材料，并以亲本品种‘C 306’（直链淀粉含量26%）作为对照，在三个籽粒发育阶段（扬花后21、28及35天，花后天数DAA）采集相关数据。所有数据设置2次生物学重复，每个重复的结果以3次技术重复的平均值±标准差（mean ± SD）表示。实验采用小麦ADP核糖基化因子（ADP Ribosylation Factor，ARF）作为归一化内参基因。数据集文件为XLSX格式，大小12 kb。