Ribosome profiling on codon optimized FIX constructs

An analysis of the structural, functional, and immunogenicity implications of F9 gene recoding. Codon optimized F9 constructs were compared for potential changes in protein folding, function, or immunogenicity. Among other techniques, ribosome profiling was used to analyze translation kinetics and possible impact of protein structure and function.本研究针对F9基因重编码的结构、功能及免疫原性影响展开分析。通过对比密码子优化后的F9基因构建体，探究其在蛋白质折叠、功能或免疫原性方面的潜在变化。本研究除采用其他多项技术手段外，还借助核糖体谱分析（ribosome profiling）技术，解析翻译动力学特征及其对蛋白质结构与功能的潜在影响。