Changes in gene expression upon treatment of SH-SY5Y cells to cisplatin

In this study we investigated the changes in mRNA expression upon treatment of SH-SY5Y cells to 10µM cisplatin for 72h. Three samples of parental SH-SY5Y cells and four samples of 72h treated SH-SY5Y neuro-blastoma cell line with 10µM CDDP were subjected to Affymetrix chip analyses. RNA quality was determined using the Agilent Bioanalyzer and the Eukaryote Total RNA Nano Chip (Ag-ilent Technologies, Hayward, CA, USA). The RNA integrity number (RIN) was measured as 9.9-10. The Affymetrix HuGene 2.0 ST and WT plus reagent kit (Affymetrix, Santa Clara, California, United States) was used following the provider’s recommendation with a start ma-terial of 100ng of total RNA.

本研究探究了SH-SY5Y细胞经10μM顺铂（cisplatin）处理72小时后，其信使RNA（mRNA）的表达变化。本研究选取3份亲本SH-SY5Y细胞样本，以及4份经10μM CDDP处理72小时的SH-SY5Y神经母细胞瘤细胞系样本，开展Affymetrix芯片分析。采用安捷伦生物分析仪（Agilent Bioanalyzer）与真核总RNA纳米芯片（Eukaryote Total RNA Nano Chip，安捷伦科技公司，美国加利福尼亚州海沃德市）完成RNA质量检测，测得RNA完整性数值（RIN）为9.9~10。按照供应商推荐的操作规范，使用Affymetrix HuGene 2.0 ST及WT Plus试剂套装（Affymetrix，美国加利福尼亚州圣克拉拉市），起始总RNA投入量为100ng。