Application of the ddPCR duplex assay on different sample matrices.

Target value: MON810 content expressed as a percentage in cp/cp ratio. aTarget value certified by the CRM provider or evaluated from the certified value of another CRM in the same series. bTarget value in cp/cp ratio attributed in the ILC-CRL-GMFF proficiency program. cTarget value estimated by qPCR, using a CRM certified in cp/cp ratio (same as “Average MON810% (qPCR)”). Average MON810% (ddPCR): Average of the MON810 content measured by ddPCR. Bias MON810% (ddPCR): Bias of the average MON810 content measured by ddPCR with the target value. Average MON810% (qPCR): Average of the MON810 content measured by qPCR. Bias MON810% (qPCR): Bias of the MON810 content measured by qPCR with the target certified value. n.a.: not applicable (value determined by qPCR is the target value).目标值：以cp/cp比值（拷贝数/拷贝数比值）表示的MON810含量百分比。 a 目标值：经CRM（Certified Reference Material，有证标准物质）提供商认证，或由同一系列其他有证标准物质的认定值推导得出。 b 以cp/cp比值表示的目标值，由ILC-CRL-GMFF能力验证计划赋值。 c 采用经cp/cp比值定值的有证标准物质，通过qPCR（quantitative polymerase chain reaction，定量聚合酶链反应）估算得到的目标值（与"Average MON810% (qPCR)"一致）。 MON810%平均值（ddPCR法）：通过ddPCR（droplet digital polymerase chain reaction，数字聚合酶链反应）测得的MON810含量的平均值。 MON810%偏倚（ddPCR法）：以目标值为参照，ddPCR法测得的MON810含量平均值的偏倚。 MON810%平均值（qPCR法）：通过qPCR测得的MON810含量的平均值。 MON810%偏倚（qPCR法）：以认定目标值为参照，qPCR法测得的MON810含量的偏倚。 n.a.：不适用（经qPCR测定的数值即为目标值）。