Chemical crosslinking of plastocyanin and the cytochrome b6f complex

In this work we investigated the role of plastocyanin (PC) phosphorylation in photosynthetic electron transfer, focusing on PC's interaction with both cytochrome b6f, (Cytb6f) and photosystem I (PSI) in Chlamydomonas reinhardtii. While the binding and electron transfer between PC and PSI are well characterized, the interaction between PC and Cytf remains less clear. Using chemical crosslinking combined with mass spectrometry, we identified two potential binding models for PC and Cytf: Side-on and Head-on.

本研究考察了莱茵衣藻（Chlamydomonas reinhardtii）中质体蓝素（plastocyanin, PC）的磷酸化在光合电子传递过程中的作用，重点聚焦于PC与细胞色素b6f（cytochrome b6f, Cytb6f）以及光系统I（photosystem I, PSI）的相互作用。尽管PC与PSI的结合过程及电子传递反应已得到充分表征，但PC与细胞色素f（cytochrome f, Cytf）之间的相互作用仍不甚明确。本研究通过化学交联法结合质谱技术，鉴定出PC与Cytf的两种潜在结合模式：侧向结合（Side-on）与正向结合（Head-on）。