Chromatin immunoprecipitation of HEK293T cells.

Chromatin immunoprecipitation of HEK293T cells was performed using fractionation-assisted native chromatin immunoprecipitation method. Specific antibodies for MLL, HBO1, PHF16, ING4, MEAF6, ENL RNAP2 non-P and RNAP2 ser-P were used for IP. The ChIPed DNAs were analyzed by deep sequencing along with the input sample.本研究采用辅助分级的天然染色质免疫共沉淀（fractionation-assisted native chromatin immunoprecipitation）方法，对HEK293T细胞开展染色质免疫共沉淀（chromatin immunoprecipitation，ChIP）实验。实验中选用针对MLL、HBO1、PHF16、ING4、MEAF6、ENL、RNAP2非磷酸化（RNAP2 non-P）及RNAP2丝氨酸磷酸化（RNAP2 ser-P）的特异性抗体进行免疫沉淀。将经染色质免疫共沉淀富集的DNA与输入对照样本一同进行深度测序（deep sequencing）分析。