Inhibition of UBE2N promoted the clearance of mutant huntingtin in HD knock-in mouse

Individual transcript analysis of subcluster-1 and subcluster-25 was performed using the heatmaps of differentially expressed genes (DEGs) from HD-/- negative (WT), vehicle-treated HD+/- (HD;Veh), and NSC697923-treated (HD;NSC) mice.The left panel displays the networks of KOG annotation analysis for DEGs in HD-/- negative mice (WT) compared to vehicle-administered HD+/- (HD;Veh) mice. The right panel illustrates the matched categorization between the HD;Veh group and NSC697923-treated HD+/- (HD;NSC) mice.The heatmaps display individual DEGs extracted from the KOG analysis of the "Posttranslational modify, protein turnover, chaperones" functional subcluster.本研究以HD纯合敲除阴性（野生型，WT）、溶剂处理的HD+/-杂合敲除（HD;Veh）及NSC697923处理的HD+/-杂合敲除（HD;NSC）小鼠的差异表达基因（differentially expressed genes, DEGs）热图作为分析基础，对亚簇1（subcluster-1）与亚簇25（subcluster-25）开展了个体转录本分析。左图展示了HD纯合敲除阴性（WT）小鼠与溶剂处理HD+/-（HD;Veh）小鼠的差异表达基因经KOG注释分析所得的网络；右图则呈现了HD;Veh组与NSC697923处理的HD+/-（HD;NSC）小鼠之间的匹配分类结果。本次展示的热图呈现了从“翻译后修饰、蛋白质周转与分子伴侣”功能亚簇的KOG分析中提取得到的个体差异表达基因。